Review



cell signaling technology 4113  (Cell Signaling Technology Inc)


Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 96
      Buy from Supplier

    Structured Review

    Cell Signaling Technology Inc cell signaling technology 4113
    Cell Signaling Technology 4113, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 313 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cell signaling technology 4113/product/Cell Signaling Technology Inc
    Average 96 stars, based on 313 article reviews
    cell signaling technology 4113 - by Bioz Stars, 2026-03
    96/100 stars

    Images



    Similar Products

    cell signaling technology 4113  (Cell Signaling Technology Inc)
    96
    Cell Signaling Technology Inc cell signaling technology 4113
    Cell Signaling Technology 4113, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cell signaling technology 4113/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    cell signaling technology 4113 - by Bioz Stars, 2026-03
    96/100 stars
    		  Buy from Supplier
    anti p stat3 4113 cell signaling technology  (Cell Signaling Technology Inc)
    96
    Cell Signaling Technology Inc anti p stat3 4113 cell signaling technology
    Anti P Stat3 4113 Cell Signaling Technology, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti p stat3 4113 cell signaling technology/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    anti p stat3 4113 cell signaling technology - by Bioz Stars, 2026-03
    96/100 stars
    		  Buy from Supplier
    foxo1 c29h4 rabbit mab cell signaling technology  (Cell Signaling Technology Inc)
    96
    Cell Signaling Technology Inc foxo1 c29h4 rabbit mab cell signaling technology
    FIGURE 6 Canonical and non-canonical leptin pathways are altered in NULL. (A) Graphical illustration of LepR signaling. Canonical (JAK2/STAT3) and non-canonical <t>PI3K/Akt/FOXO1</t> and ERK pathways are indicated. (B) Representative Western blot and densitometric quantification of the long isoform leptin receptor (LepR) in hypothalamic lysates of WT and NULL mice. (C) Representative Western blot of STAT3 phosphorylated at Y705 (pSTAT3) and total STAT3 protein (STAT3) in hypothalamic lysates of WT and NULL mice in absence/presence of leptin. (D) Densitometric quantifications of the fraction of pSTAT3/STAP calculated from Westerns as that in (C). (E) Representative Western blot of phosphorylated FOXO1 at S256 (pFOXO1) and total <t>FOXO1</t> <t>protein</t> (FOXO1) in hypothalamic lysates of WT and NULL mice in the absence/presence of leptin. (F) Densitometric quantifications of pFOXO1/FOXO1 calculated from Westerns as those in (E). (G) ELISA quantifications of phosphorylated Akt at S473 (pAkt) over total Akt protein (pAkt/Akt) in hypothalamic lysates of WT and NULL mice in the absence/presence of leptin. (H) Representative Western blots of phosphorylated ERK1/2 at T202/T204 (pERK) and total ERK1/2 (ERK) protein in hypothalamic lysates of WT and NULL mice in the absence/presence of leptin and corresponding densitometric quantifications of the fraction of phosphorylated ERK1/2 over total ERK1/2 (pERK/ERK). (I) ELISA quantifications of αMSH in hypothalamic lysates of WT and NULL mice in the absence/presence of leptin. Western blot loading controls were actin and Bradford colorimetric assay. Animals were injected with either vehicle or 1.0 mg/kg body weight leptin and sacrificed 90 min later. *p < .05 (ANOVA with Tukey's post hoc).
    Foxo1 C29h4 Rabbit Mab Cell Signaling Technology, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/foxo1 c29h4 rabbit mab cell signaling technology/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    foxo1 c29h4 rabbit mab cell signaling technology - by Bioz Stars, 2026-03
    96/100 stars
    		  Buy from Supplier
    p stat3 cell signaling technology 4113s  (Cell Signaling Technology Inc)
    96
    Cell Signaling Technology Inc p stat3 cell signaling technology 4113s
    FIGURE 6 Canonical and non-canonical leptin pathways are altered in NULL. (A) Graphical illustration of LepR signaling. Canonical (JAK2/STAT3) and non-canonical <t>PI3K/Akt/FOXO1</t> and ERK pathways are indicated. (B) Representative Western blot and densitometric quantification of the long isoform leptin receptor (LepR) in hypothalamic lysates of WT and NULL mice. (C) Representative Western blot of STAT3 phosphorylated at Y705 (pSTAT3) and total STAT3 protein (STAT3) in hypothalamic lysates of WT and NULL mice in absence/presence of leptin. (D) Densitometric quantifications of the fraction of pSTAT3/STAP calculated from Westerns as that in (C). (E) Representative Western blot of phosphorylated FOXO1 at S256 (pFOXO1) and total <t>FOXO1</t> <t>protein</t> (FOXO1) in hypothalamic lysates of WT and NULL mice in the absence/presence of leptin. (F) Densitometric quantifications of pFOXO1/FOXO1 calculated from Westerns as those in (E). (G) ELISA quantifications of phosphorylated Akt at S473 (pAkt) over total Akt protein (pAkt/Akt) in hypothalamic lysates of WT and NULL mice in the absence/presence of leptin. (H) Representative Western blots of phosphorylated ERK1/2 at T202/T204 (pERK) and total ERK1/2 (ERK) protein in hypothalamic lysates of WT and NULL mice in the absence/presence of leptin and corresponding densitometric quantifications of the fraction of phosphorylated ERK1/2 over total ERK1/2 (pERK/ERK). (I) ELISA quantifications of αMSH in hypothalamic lysates of WT and NULL mice in the absence/presence of leptin. Western blot loading controls were actin and Bradford colorimetric assay. Animals were injected with either vehicle or 1.0 mg/kg body weight leptin and sacrificed 90 min later. *p < .05 (ANOVA with Tukey's post hoc).
    P Stat3 Cell Signaling Technology 4113s, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p stat3 cell signaling technology 4113s/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    p stat3 cell signaling technology 4113s - by Bioz Stars, 2026-03
    96/100 stars
    		  Buy from Supplier
    4113 cell signaling technology akt 9272 cell signaling technology  (Cell Signaling Technology Inc)
    99
    Cell Signaling Technology Inc 4113 cell signaling technology akt 9272 cell signaling technology
    FIGURE 6 Canonical and non-canonical leptin pathways are altered in NULL. (A) Graphical illustration of LepR signaling. Canonical (JAK2/STAT3) and non-canonical <t>PI3K/Akt/FOXO1</t> and ERK pathways are indicated. (B) Representative Western blot and densitometric quantification of the long isoform leptin receptor (LepR) in hypothalamic lysates of WT and NULL mice. (C) Representative Western blot of STAT3 phosphorylated at Y705 (pSTAT3) and total STAT3 protein (STAT3) in hypothalamic lysates of WT and NULL mice in absence/presence of leptin. (D) Densitometric quantifications of the fraction of pSTAT3/STAP calculated from Westerns as that in (C). (E) Representative Western blot of phosphorylated FOXO1 at S256 (pFOXO1) and total <t>FOXO1</t> <t>protein</t> (FOXO1) in hypothalamic lysates of WT and NULL mice in the absence/presence of leptin. (F) Densitometric quantifications of pFOXO1/FOXO1 calculated from Westerns as those in (E). (G) ELISA quantifications of phosphorylated Akt at S473 (pAkt) over total Akt protein (pAkt/Akt) in hypothalamic lysates of WT and NULL mice in the absence/presence of leptin. (H) Representative Western blots of phosphorylated ERK1/2 at T202/T204 (pERK) and total ERK1/2 (ERK) protein in hypothalamic lysates of WT and NULL mice in the absence/presence of leptin and corresponding densitometric quantifications of the fraction of phosphorylated ERK1/2 over total ERK1/2 (pERK/ERK). (I) ELISA quantifications of αMSH in hypothalamic lysates of WT and NULL mice in the absence/presence of leptin. Western blot loading controls were actin and Bradford colorimetric assay. Animals were injected with either vehicle or 1.0 mg/kg body weight leptin and sacrificed 90 min later. *p < .05 (ANOVA with Tukey's post hoc).
    4113 Cell Signaling Technology Akt 9272 Cell Signaling Technology, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/4113 cell signaling technology akt 9272 cell signaling technology/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
    4113 cell signaling technology akt 9272 cell signaling technology - by Bioz Stars, 2026-03
    99/100 stars
    		  Buy from Supplier
    p py705 cell signaling technology function  (Cell Signaling Technology Inc)
    96
    Cell Signaling Technology Inc p py705 cell signaling technology function
    FIGURE 6 Canonical and non-canonical leptin pathways are altered in NULL. (A) Graphical illustration of LepR signaling. Canonical (JAK2/STAT3) and non-canonical <t>PI3K/Akt/FOXO1</t> and ERK pathways are indicated. (B) Representative Western blot and densitometric quantification of the long isoform leptin receptor (LepR) in hypothalamic lysates of WT and NULL mice. (C) Representative Western blot of STAT3 phosphorylated at Y705 (pSTAT3) and total STAT3 protein (STAT3) in hypothalamic lysates of WT and NULL mice in absence/presence of leptin. (D) Densitometric quantifications of the fraction of pSTAT3/STAP calculated from Westerns as that in (C). (E) Representative Western blot of phosphorylated FOXO1 at S256 (pFOXO1) and total <t>FOXO1</t> <t>protein</t> (FOXO1) in hypothalamic lysates of WT and NULL mice in the absence/presence of leptin. (F) Densitometric quantifications of pFOXO1/FOXO1 calculated from Westerns as those in (E). (G) ELISA quantifications of phosphorylated Akt at S473 (pAkt) over total Akt protein (pAkt/Akt) in hypothalamic lysates of WT and NULL mice in the absence/presence of leptin. (H) Representative Western blots of phosphorylated ERK1/2 at T202/T204 (pERK) and total ERK1/2 (ERK) protein in hypothalamic lysates of WT and NULL mice in the absence/presence of leptin and corresponding densitometric quantifications of the fraction of phosphorylated ERK1/2 over total ERK1/2 (pERK/ERK). (I) ELISA quantifications of αMSH in hypothalamic lysates of WT and NULL mice in the absence/presence of leptin. Western blot loading controls were actin and Bradford colorimetric assay. Animals were injected with either vehicle or 1.0 mg/kg body weight leptin and sacrificed 90 min later. *p < .05 (ANOVA with Tukey's post hoc).
    P Py705 Cell Signaling Technology Function, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p py705 cell signaling technology function/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    p py705 cell signaling technology function - by Bioz Stars, 2026-03
    96/100 stars
    		  Buy from Supplier
    custom 155gd prps6 ps235 ps236 n7 548 cell signaling technology function  (Cell Signaling Technology Inc)
    96
    Cell Signaling Technology Inc custom 155gd prps6 ps235 ps236 n7 548 cell signaling technology function
    FIGURE 6 Canonical and non-canonical leptin pathways are altered in NULL. (A) Graphical illustration of LepR signaling. Canonical (JAK2/STAT3) and non-canonical <t>PI3K/Akt/FOXO1</t> and ERK pathways are indicated. (B) Representative Western blot and densitometric quantification of the long isoform leptin receptor (LepR) in hypothalamic lysates of WT and NULL mice. (C) Representative Western blot of STAT3 phosphorylated at Y705 (pSTAT3) and total STAT3 protein (STAT3) in hypothalamic lysates of WT and NULL mice in absence/presence of leptin. (D) Densitometric quantifications of the fraction of pSTAT3/STAP calculated from Westerns as that in (C). (E) Representative Western blot of phosphorylated FOXO1 at S256 (pFOXO1) and total <t>FOXO1</t> <t>protein</t> (FOXO1) in hypothalamic lysates of WT and NULL mice in the absence/presence of leptin. (F) Densitometric quantifications of pFOXO1/FOXO1 calculated from Westerns as those in (E). (G) ELISA quantifications of phosphorylated Akt at S473 (pAkt) over total Akt protein (pAkt/Akt) in hypothalamic lysates of WT and NULL mice in the absence/presence of leptin. (H) Representative Western blots of phosphorylated ERK1/2 at T202/T204 (pERK) and total ERK1/2 (ERK) protein in hypothalamic lysates of WT and NULL mice in the absence/presence of leptin and corresponding densitometric quantifications of the fraction of phosphorylated ERK1/2 over total ERK1/2 (pERK/ERK). (I) ELISA quantifications of αMSH in hypothalamic lysates of WT and NULL mice in the absence/presence of leptin. Western blot loading controls were actin and Bradford colorimetric assay. Animals were injected with either vehicle or 1.0 mg/kg body weight leptin and sacrificed 90 min later. *p < .05 (ANOVA with Tukey's post hoc).
    Custom 155gd Prps6 Ps235 Ps236 N7 548 Cell Signaling Technology Function, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/custom 155gd prps6 ps235 ps236 n7 548 cell signaling technology function/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    custom 155gd prps6 ps235 ps236 n7 548 cell signaling technology function - by Bioz Stars, 2026-03
    96/100 stars
    		  Buy from Supplier
    cell signaling technology 4113s  (Cell Signaling Technology Inc)
    96
    Cell Signaling Technology Inc cell signaling technology 4113s
    FIGURE 6 Canonical and non-canonical leptin pathways are altered in NULL. (A) Graphical illustration of LepR signaling. Canonical (JAK2/STAT3) and non-canonical <t>PI3K/Akt/FOXO1</t> and ERK pathways are indicated. (B) Representative Western blot and densitometric quantification of the long isoform leptin receptor (LepR) in hypothalamic lysates of WT and NULL mice. (C) Representative Western blot of STAT3 phosphorylated at Y705 (pSTAT3) and total STAT3 protein (STAT3) in hypothalamic lysates of WT and NULL mice in absence/presence of leptin. (D) Densitometric quantifications of the fraction of pSTAT3/STAP calculated from Westerns as that in (C). (E) Representative Western blot of phosphorylated FOXO1 at S256 (pFOXO1) and total <t>FOXO1</t> <t>protein</t> (FOXO1) in hypothalamic lysates of WT and NULL mice in the absence/presence of leptin. (F) Densitometric quantifications of pFOXO1/FOXO1 calculated from Westerns as those in (E). (G) ELISA quantifications of phosphorylated Akt at S473 (pAkt) over total Akt protein (pAkt/Akt) in hypothalamic lysates of WT and NULL mice in the absence/presence of leptin. (H) Representative Western blots of phosphorylated ERK1/2 at T202/T204 (pERK) and total ERK1/2 (ERK) protein in hypothalamic lysates of WT and NULL mice in the absence/presence of leptin and corresponding densitometric quantifications of the fraction of phosphorylated ERK1/2 over total ERK1/2 (pERK/ERK). (I) ELISA quantifications of αMSH in hypothalamic lysates of WT and NULL mice in the absence/presence of leptin. Western blot loading controls were actin and Bradford colorimetric assay. Animals were injected with either vehicle or 1.0 mg/kg body weight leptin and sacrificed 90 min later. *p < .05 (ANOVA with Tukey's post hoc).
    Cell Signaling Technology 4113s, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cell signaling technology 4113s/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    cell signaling technology 4113s - by Bioz Stars, 2026-03
    96/100 stars
    		  Buy from Supplier
    4113 cell signaling technology  (Cell Signaling Technology Inc)
    96
    Cell Signaling Technology Inc 4113 cell signaling technology
    FIGURE 6 Canonical and non-canonical leptin pathways are altered in NULL. (A) Graphical illustration of LepR signaling. Canonical (JAK2/STAT3) and non-canonical <t>PI3K/Akt/FOXO1</t> and ERK pathways are indicated. (B) Representative Western blot and densitometric quantification of the long isoform leptin receptor (LepR) in hypothalamic lysates of WT and NULL mice. (C) Representative Western blot of STAT3 phosphorylated at Y705 (pSTAT3) and total STAT3 protein (STAT3) in hypothalamic lysates of WT and NULL mice in absence/presence of leptin. (D) Densitometric quantifications of the fraction of pSTAT3/STAP calculated from Westerns as that in (C). (E) Representative Western blot of phosphorylated FOXO1 at S256 (pFOXO1) and total <t>FOXO1</t> <t>protein</t> (FOXO1) in hypothalamic lysates of WT and NULL mice in the absence/presence of leptin. (F) Densitometric quantifications of pFOXO1/FOXO1 calculated from Westerns as those in (E). (G) ELISA quantifications of phosphorylated Akt at S473 (pAkt) over total Akt protein (pAkt/Akt) in hypothalamic lysates of WT and NULL mice in the absence/presence of leptin. (H) Representative Western blots of phosphorylated ERK1/2 at T202/T204 (pERK) and total ERK1/2 (ERK) protein in hypothalamic lysates of WT and NULL mice in the absence/presence of leptin and corresponding densitometric quantifications of the fraction of phosphorylated ERK1/2 over total ERK1/2 (pERK/ERK). (I) ELISA quantifications of αMSH in hypothalamic lysates of WT and NULL mice in the absence/presence of leptin. Western blot loading controls were actin and Bradford colorimetric assay. Animals were injected with either vehicle or 1.0 mg/kg body weight leptin and sacrificed 90 min later. *p < .05 (ANOVA with Tukey's post hoc).
    4113 Cell Signaling Technology, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/4113 cell signaling technology/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    4113 cell signaling technology - by Bioz Stars, 2026-03
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    FIGURE 6 Canonical and non-canonical leptin pathways are altered in NULL. (A) Graphical illustration of LepR signaling. Canonical (JAK2/STAT3) and non-canonical PI3K/Akt/FOXO1 and ERK pathways are indicated. (B) Representative Western blot and densitometric quantification of the long isoform leptin receptor (LepR) in hypothalamic lysates of WT and NULL mice. (C) Representative Western blot of STAT3 phosphorylated at Y705 (pSTAT3) and total STAT3 protein (STAT3) in hypothalamic lysates of WT and NULL mice in absence/presence of leptin. (D) Densitometric quantifications of the fraction of pSTAT3/STAP calculated from Westerns as that in (C). (E) Representative Western blot of phosphorylated FOXO1 at S256 (pFOXO1) and total FOXO1 protein (FOXO1) in hypothalamic lysates of WT and NULL mice in the absence/presence of leptin. (F) Densitometric quantifications of pFOXO1/FOXO1 calculated from Westerns as those in (E). (G) ELISA quantifications of phosphorylated Akt at S473 (pAkt) over total Akt protein (pAkt/Akt) in hypothalamic lysates of WT and NULL mice in the absence/presence of leptin. (H) Representative Western blots of phosphorylated ERK1/2 at T202/T204 (pERK) and total ERK1/2 (ERK) protein in hypothalamic lysates of WT and NULL mice in the absence/presence of leptin and corresponding densitometric quantifications of the fraction of phosphorylated ERK1/2 over total ERK1/2 (pERK/ERK). (I) ELISA quantifications of αMSH in hypothalamic lysates of WT and NULL mice in the absence/presence of leptin. Western blot loading controls were actin and Bradford colorimetric assay. Animals were injected with either vehicle or 1.0 mg/kg body weight leptin and sacrificed 90 min later. *p < .05 (ANOVA with Tukey's post hoc).

    Journal: The FASEB Journal

    Article Title: KCNB1‐Leptin receptor complexes couple electric and endocrine function in the melanocortin neurons of the hypothalamus

    doi: 10.1096/fj.202401931r

    Figure Lengend Snippet: FIGURE 6 Canonical and non-canonical leptin pathways are altered in NULL. (A) Graphical illustration of LepR signaling. Canonical (JAK2/STAT3) and non-canonical PI3K/Akt/FOXO1 and ERK pathways are indicated. (B) Representative Western blot and densitometric quantification of the long isoform leptin receptor (LepR) in hypothalamic lysates of WT and NULL mice. (C) Representative Western blot of STAT3 phosphorylated at Y705 (pSTAT3) and total STAT3 protein (STAT3) in hypothalamic lysates of WT and NULL mice in absence/presence of leptin. (D) Densitometric quantifications of the fraction of pSTAT3/STAP calculated from Westerns as that in (C). (E) Representative Western blot of phosphorylated FOXO1 at S256 (pFOXO1) and total FOXO1 protein (FOXO1) in hypothalamic lysates of WT and NULL mice in the absence/presence of leptin. (F) Densitometric quantifications of pFOXO1/FOXO1 calculated from Westerns as those in (E). (G) ELISA quantifications of phosphorylated Akt at S473 (pAkt) over total Akt protein (pAkt/Akt) in hypothalamic lysates of WT and NULL mice in the absence/presence of leptin. (H) Representative Western blots of phosphorylated ERK1/2 at T202/T204 (pERK) and total ERK1/2 (ERK) protein in hypothalamic lysates of WT and NULL mice in the absence/presence of leptin and corresponding densitometric quantifications of the fraction of phosphorylated ERK1/2 over total ERK1/2 (pERK/ERK). (I) ELISA quantifications of αMSH in hypothalamic lysates of WT and NULL mice in the absence/presence of leptin. Western blot loading controls were actin and Bradford colorimetric assay. Animals were injected with either vehicle or 1.0 mg/kg body weight leptin and sacrificed 90 min later. *p < .05 (ANOVA with Tukey's post hoc).

    Article Snippet: Reagent or resource Source Identifier Antibodies POMC (D3R1U) Rabbit mAb Cell Signaling Technology #23499 c- Fos (9F6) Rabbit mAb Cell Signaling Technology #2250 Stat3 (124H6) Mouse mAb Cell Signaling Technology #9139 Phospho- Stat3 (Tyr705) (M9C6) Mouse mAb Cell Signaling Technology #4113 FoxO1 (C29H4) Rabbit mAb Cell Signaling Technology #2880 Phospho- FoxO1 (Ser256) (E1F7T) Rabbit mAb Cell Signaling Technology #84192 p44/42 MAP kinase (phosphorylated Erk1/2) Cell Signaling Technology Cat# 9101 p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb Cell Signaling Technology Cat# 4695 Integrin beta- 5 (D24A5) Rabbit mAb Cell Signaling Technology Cat# 3629 Integrin beta- 1 antibody Cell Signaling Technology Cat #4706 Alpha MSH polyclonal rabbit Bioss Antibodies bs- 1848R AgRP polyclonal antibody Thermo Fisher PA5- 118934 Leptin receptor polyclonal antibody Thermo Fisher PA1- 053 Leptin receptor recombinant Rabbit monoclonal antibody Thermo Fisher MA5- 32685 (NIH), Grant/Award Number: R01AG060919; National Science Foundation (NSF), Grant/Award Number: 2030348 upon leptin stimulation.

    Techniques: Western Blot, Enzyme-linked Immunosorbent Assay, Colorimetric Assay, Injection